Tomosyn functions as a PKCδ-regulated fusion clamp in mast cell degranulation

Mechanism of Peptide-induced Mast Cell Degranulation Translocation and Patch-Clamp Studies

Substance P and other polycationic peptides are thought to stimulate mast cell degranulation via direct activation of G proteins. We investigated the ability of extracellularly applied substance P to translocate into mast cells and the ability of intracellularly applied substance P to stimulate degranulation. In addition, we studied by reverse transcription–-PCR whether substance Pspecific rece...

Mechanism of Peptide-induced Mast Cell Degranulation

Substance P and other polycationic peptides are thought to stimulate mast cell degranulation via direct activation of G proteins. We investigated the ability of extracellularly applied substance P to translocate into mast cells and the ability of intracellularly applied substance P to stimulate degranulation. In addition, we studied by reverse transcription--PCR whether substance P-specific rec...

RIII-dependent Mast Cell Degranulation and Anaphylaxis

In mouse mast cells, both Fc e RI and Fc g RIII are abg 2 tetrameric complexes in which different a chains confer IgE or IgG ligand recognition while the signaling FcR b and g chains are identical. We used primarily noninvasive techniques (changes in body temperature, dye extravasation) to assess systemic anaphylactic responses in nonanesthetized wild-type, Fc e RI a chain 2 / 2 and FcR g chain...

Inosine Binding Stimulates Mast Cell Degranulation

We investigated the mechanism by which inosine, a metabolite of adenosine that accumulates to . 1 mM levels in ischemic tissues, triggers mast cell degranulation. Inosine was found to do the following: ( a ) compete for [ 125 I] N 6 -aminobenzyladenosine binding to recombinant rat A 3 adenosine receptors (A 3 AR) with an IC 50 of 25 6 6 m M; ( b ) not bind to A 1 or A 2A ARs; ( c ) bind to newl...

Degranulation Age-Induced Reprogramming of Mast Cell